ELISA, WB, IF
Monoclonal
NI 158
IgG1 kappa
unconjugated
N° du produit ABIN457824
Aperçu rapide pour Souris anti-Humain IgD Anticorps (ABIN457824)
Antigène
IgD
Clonalité
Monoclonal
Application
ELISA, Western Blotting (WB), Immunofluorescence (IF)
Clone
NI 158
Isotype
IgG1 kappa
Reactivité
Humain
Hôte
Souris
Specificité
The reactivity of the antiserum is restricted to the IgD molecule as tested in ELISA, immunoblotting, immunoprecipitation. Precipitation takes place under special test conditions in the presence of polyethylene glycol. Purified monoclonal mouse antibody to human IgD, isotype specific
Attributs du produit
Purified monoclonal mouse antibody to human IgD, isotype specific
Purification
Purified
Immunogène
Highly purified monoclonal IgD isolated from human serum
To identify the presence of IgD in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immuno-peroxidase and immunofluorescence staining of cytoplasmic IgD immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:100, in ELISA from 1:25 upwards, in Western blotting from 1:50 upwards. These data should be interpreted as general recommendations only.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitute the lyophilized ascites by adding 0. 5 mL sterile distilled water.
Concentration
0.4 mg/mL
Buffer
Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2).
Conseil sur la manipulation
Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.
Stock
4 °C/-20 °C
Stockage commentaire
The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.